Human Neoplastic Cells in Tissue
نویسندگان
چکیده
The establishment and cultivation o ľ two human neoplastic cell lines is described. The cell line B-5GT was derived from bono giant cell tumor and B-6F8 from poorly differentiated fibrosarcoma. In comparison to the normal skin fibroblasts both cell lines have a potential for "indefinite" multiplication in vitro and 1 hoy exhibit growth properties which are asso ciated with malignant transformation. The parameters investigated included cell morphology, chromosome characteristics, terminal cell density, growth pattern, residual DNA synthesis and growth in soft agar. Both cell lines exhibited human karyotype with aneuploidy and differed in their karyotype from each other.
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